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Identifying mind-body practices that show physiological or clear clinical benefits with appropriate biomarkers may lead to the refinement of certain techniques so that they become more efficient, less time-consuming, more effective, and better suited for the treatment of specific conditions. Also, mind-body practices can be used as noninvasive probes to explore fundamental neurophysiological processes and anatomic networks using brain imaging and other advanced technologies. Understanding the physiological changes, clinical benefits, adverse effects and contraindications associated with these practices will support the inclusion of mind-body treatments in mainstream medicine.